Contact an Account ManagerDetermination of vancomycin in serum and tissues by column liquid chromatography using solid-phase extraction
Summary
Type: HPLC
Application #: 103690
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: UV, 229nm
Conditions: eluent: 5mM KH2PO4, adjusted to pH 2.8 with orthophosphoric acid - methanol (78:22, v/v)
flow rate 1.5ml/min
Authors: Bauchet J
Source: J Chromatogr 414 (1987) 472 - 476
Details
Legend: Fig. 1. Chromatograms of A) a drug-free blank serum sample, B) a calibration
standard containing 20µg/ml vancomycin and 5µg/ml trimethoprim, C) a serum
sample from subject 2 drawn at the end of card